phospho-p38 (pp38) Search Results


anti p38 mapk phospho thr180 tyr182 antibody  (Revvity)
92
Revvity anti p38 mapk phospho thr180 tyr182 antibody
<t>IL-33-ST2L-p38</t> MAPK signaling was impeded in kidney ILC2s treated with HIF-PHD inhibitors. ( A ) ILC2s were sorted from kidneys of pooled 6 mice, and cultured with IL-2 and IL-7 for 3 days. Then IL-33 was added to stimulate ILC2s for further 3 days, and HIF-PHD inhibitors were added for the last 24 h. Relative levels of mRNA expression for ST2L in kidney ILC2s treated with DMSO, GSK360A, and FG-4592. ( B ) Sorted kidney ILC2s were stimulated by 50 ng/ml recombinant murine IL-33 or 50 ng/ml PMA and 500 ng/ml ionomycin for 3 h in the presence of brefeldin A. ST2L MFI was analyzed by FACS. ( C ) Sorted kidney ILC2s were cultured with IL-2 and IL-7 for 3 days, and half of the media was changed with fresh media containing IL-2 and IL-7. After a further 3 days, ILC2s were cultured in cytokine-free conditions for 4 h in the presence of HIF-PHD inhibitors, and then ILC2s were stimulated with 10 ng/ml recombinant murine IL-33 for 15 min. Phosphorylated <t>p38</t> <t>MAPK</t> <t>(Thr180/Tyr182)</t> was assessed by FACS. Representative histograms are shown for phosphorylated p38 MAPK in kidney ILC2s treated with DMSO (gray area), GSK360A (dashed line), FG-4592 (long-dashed line), IL-33 non-stimulated (solid line), and isotype control (black area). ( D ) The graph shows the frequency of phosphorylated p38 MAPK. We used the pooled kidney from 6 mice as n = 1, and data shown are pooled from two (A,B) (n = 6) or three (D) (n = 9) independent experiments. Error bars show SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.
Anti P38 Mapk Phospho Thr180 Tyr182 Antibody, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p38 mapk phospho thr180 tyr182 antibody/product/Revvity
Average 92 stars, based on 1 article reviews
anti p38 mapk phospho thr180 tyr182 antibody - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier
dual phospho-p38 mapk (pp38 mapk) antibody  (Stressgen Biotechnologies)
90
Stressgen Biotechnologies dual phospho-p38 mapk (pp38 mapk) antibody
<t>IL-33-ST2L-p38</t> MAPK signaling was impeded in kidney ILC2s treated with HIF-PHD inhibitors. ( A ) ILC2s were sorted from kidneys of pooled 6 mice, and cultured with IL-2 and IL-7 for 3 days. Then IL-33 was added to stimulate ILC2s for further 3 days, and HIF-PHD inhibitors were added for the last 24 h. Relative levels of mRNA expression for ST2L in kidney ILC2s treated with DMSO, GSK360A, and FG-4592. ( B ) Sorted kidney ILC2s were stimulated by 50 ng/ml recombinant murine IL-33 or 50 ng/ml PMA and 500 ng/ml ionomycin for 3 h in the presence of brefeldin A. ST2L MFI was analyzed by FACS. ( C ) Sorted kidney ILC2s were cultured with IL-2 and IL-7 for 3 days, and half of the media was changed with fresh media containing IL-2 and IL-7. After a further 3 days, ILC2s were cultured in cytokine-free conditions for 4 h in the presence of HIF-PHD inhibitors, and then ILC2s were stimulated with 10 ng/ml recombinant murine IL-33 for 15 min. Phosphorylated <t>p38</t> <t>MAPK</t> <t>(Thr180/Tyr182)</t> was assessed by FACS. Representative histograms are shown for phosphorylated p38 MAPK in kidney ILC2s treated with DMSO (gray area), GSK360A (dashed line), FG-4592 (long-dashed line), IL-33 non-stimulated (solid line), and isotype control (black area). ( D ) The graph shows the frequency of phosphorylated p38 MAPK. We used the pooled kidney from 6 mice as n = 1, and data shown are pooled from two (A,B) (n = 6) or three (D) (n = 9) independent experiments. Error bars show SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.
Dual Phospho P38 Mapk (Pp38 Mapk) Antibody, supplied by Stressgen Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dual phospho-p38 mapk (pp38 mapk) antibody/product/Stressgen Biotechnologies
Average 90 stars, based on 1 article reviews
dual phospho-p38 mapk (pp38 mapk) antibody - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


IL-33-ST2L-p38 MAPK signaling was impeded in kidney ILC2s treated with HIF-PHD inhibitors. ( A ) ILC2s were sorted from kidneys of pooled 6 mice, and cultured with IL-2 and IL-7 for 3 days. Then IL-33 was added to stimulate ILC2s for further 3 days, and HIF-PHD inhibitors were added for the last 24 h. Relative levels of mRNA expression for ST2L in kidney ILC2s treated with DMSO, GSK360A, and FG-4592. ( B ) Sorted kidney ILC2s were stimulated by 50 ng/ml recombinant murine IL-33 or 50 ng/ml PMA and 500 ng/ml ionomycin for 3 h in the presence of brefeldin A. ST2L MFI was analyzed by FACS. ( C ) Sorted kidney ILC2s were cultured with IL-2 and IL-7 for 3 days, and half of the media was changed with fresh media containing IL-2 and IL-7. After a further 3 days, ILC2s were cultured in cytokine-free conditions for 4 h in the presence of HIF-PHD inhibitors, and then ILC2s were stimulated with 10 ng/ml recombinant murine IL-33 for 15 min. Phosphorylated p38 MAPK (Thr180/Tyr182) was assessed by FACS. Representative histograms are shown for phosphorylated p38 MAPK in kidney ILC2s treated with DMSO (gray area), GSK360A (dashed line), FG-4592 (long-dashed line), IL-33 non-stimulated (solid line), and isotype control (black area). ( D ) The graph shows the frequency of phosphorylated p38 MAPK. We used the pooled kidney from 6 mice as n = 1, and data shown are pooled from two (A,B) (n = 6) or three (D) (n = 9) independent experiments. Error bars show SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Scientific Reports

Article Title: HIF-PHD inhibitor regulates the function of group2 innate lymphoid cells and polarization of M2 macrophages

doi: 10.1038/s41598-023-29161-3

Figure Lengend Snippet: IL-33-ST2L-p38 MAPK signaling was impeded in kidney ILC2s treated with HIF-PHD inhibitors. ( A ) ILC2s were sorted from kidneys of pooled 6 mice, and cultured with IL-2 and IL-7 for 3 days. Then IL-33 was added to stimulate ILC2s for further 3 days, and HIF-PHD inhibitors were added for the last 24 h. Relative levels of mRNA expression for ST2L in kidney ILC2s treated with DMSO, GSK360A, and FG-4592. ( B ) Sorted kidney ILC2s were stimulated by 50 ng/ml recombinant murine IL-33 or 50 ng/ml PMA and 500 ng/ml ionomycin for 3 h in the presence of brefeldin A. ST2L MFI was analyzed by FACS. ( C ) Sorted kidney ILC2s were cultured with IL-2 and IL-7 for 3 days, and half of the media was changed with fresh media containing IL-2 and IL-7. After a further 3 days, ILC2s were cultured in cytokine-free conditions for 4 h in the presence of HIF-PHD inhibitors, and then ILC2s were stimulated with 10 ng/ml recombinant murine IL-33 for 15 min. Phosphorylated p38 MAPK (Thr180/Tyr182) was assessed by FACS. Representative histograms are shown for phosphorylated p38 MAPK in kidney ILC2s treated with DMSO (gray area), GSK360A (dashed line), FG-4592 (long-dashed line), IL-33 non-stimulated (solid line), and isotype control (black area). ( D ) The graph shows the frequency of phosphorylated p38 MAPK. We used the pooled kidney from 6 mice as n = 1, and data shown are pooled from two (A,B) (n = 6) or three (D) (n = 9) independent experiments. Error bars show SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: To detect phosphorylated-p38 MAPK, ILC2s were cultured with cytokine free condition for 4 h, and stimulated with 10 ng/ml IL-33 for 15 min, and immediately fixed/permeabilized by pre-chilled methanol, and stained with anti-p38 MAPK Phospho (Thr180/Tyr182) antibody (Biolegend).

Techniques: Cell Culture, Expressing, Recombinant, Control